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Evaluation of the protective effect of vitamin E on sperm parameters in the mice exposed to zolpidem
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Marziye Karimi1    , Mohammad Taghi Mohammad Taghi 2, Zahra Tootian3 , Simin Fazelipour4 , Mohammad Babaei5 |
1- PhD Student of Comparative Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2- Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , Sheybani@ut.ac.ir
3- Department of Anatomy & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4- Department of Anatomy, Faculty of Tehran Medical Science, Islamic Azad University, Tehran, Iran
5- Department of Clinical Sciences, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran |
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Abstract: (208 Views) |
Background: Due to lifestyle changes and increased anxiety consumption of zolpidem has increased among young people. Zolpidem is the second sleeping medication used in the world. the harmful effects of this drug on the reproductive system have been proven. The present study was conducted to evaluate the protective effects of vitamin E against zolpidem injuries on male reproductive system.
Materials and methods: In this experimental study forty eight adult male mice NMRI strain at a mean weight of 25±5 grams were divided into one control and seven experimental groups. Zolpidem was prepared in distilled water. The control group received distilled water (as solvent of zolpidem) The groups 1, 2 and 3 received zolpidem at the doses 5, 10 and 20 (mg/kg of body weight). The group of 4 received (100 IU/kg of body weight) vitamin E and the groups of 5,6 and 7 received Zolpidem+ vitamin E for 35 days. One day after the last gavage, the treatment groups were sacrificed, the hearts were dissected and blood samples were obtained. Then the sperm parameters MDA, TAC and testosterone were evaluated.
Results: Significant reduction in the count, motility, number of live and mature sperms at high doses were observed. DNA damage was increased, which vitamin E could result in improvement. The total antioxidant capacity and testosterone levels decreased while the amount of malondialdehyde level in the serum increased. Administration of Vitamin E improved MDA, TAC and testosterone level.
Conclusion: Zolpidem can increase the free radicals and weakens the body's antioxidant defense system, zolpidem causes disorders related to sperm parameters in high doses and therefore is able to impair the physiological function of male reproductive system. On the other hand, vitamin E, which is a fat-soluble antioxidant, can improve the damage and eliminate reproductive disorders.
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| Keywords: Vitamin E, Zolpidem, Sperm, MDA, Testosterone |
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Full-Text [PDF 891 kb]
(73 Downloads)
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Semi-pilot: Experimental |
Subject:
Anatomy
Received: 2023/06/13 | Accepted: 2023/08/28 | Published: 2024/02/29
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